Bacteria:Article Title: Method for producing aldehyde
Article Snippet: .. Corynebacterium acetoacidophilum ATCC 13870 Corynebacterium acetoglutamicum ATCC 15806 Corynebacterium alkanolyticum ATCC 21511 Corynebacterium callunae ATCC 15991 Corynebacterium crenatum AS1.542 Corynebacterium glutamicum ATCC 13020, ATCC 13032, ATCC 13060, ATCC 13869, FERM BP-734 Corynebacterium lilium ATCC 15990 Corynebacterium melassecola ATCC 17965 Corynebacterium efficiens (Corynebacterium thermoaminogenes) AJ12340 (FERM BP-1539) Corynebacterium herculis ATCC 13868 Brevibacterium divaricatum (Corynebacterium glutamicum) ATCC 14020 Brevibacterium flavum (Corynebacterium glutamicum) ATCC 13826, ATCC 14067, AJ12418 (FERM BP-2205) Brevibacterium immariophilum ATCC 14068 Brevibacterium lactofermentum (Corynebacterium glutamicum) ATCC 13869 (2256 strain) Brevibacterium roseum ATCC 13825 Brevibacterium saccharolyticum ATCC 14066 Brevibacterium thiogenitalis ATCC 19240 Corynebacterium ammoniagenes (Corynebacterium stationis) ATCC 6871, ATCC 6872 Brevibacterium album ATCC 15111 Brevibacterium cerinum ATCC 15112 Microbacterium ammoniaphilum ATCC 15354 The coryneform bacteria can include bacteria that had previously been classified into the genus Brevibacterium, but have since been united into the genus Corynebacterium (Int. ..
Plasmid Preparation:Article Title: Single-Base Genome Editing in Corynebacterium glutamicum with the Help of Negative Selection by Target-Mismatched CRISPR/Cpf1
Article Snippet: .. Name Characteristics Reference or source Bacterial strains Escherichia coli DH5α F-eNDA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17 (rK-mK),λ– Laboratory stock Corynebacterium glutamicum ATCC 13869 Wild type American Type Culture Collection (ATCC) HK1220 Single cross-over integration of the cpf1 gene in ATCC 13869 This study Plasmids pK18mobsacB sacB, lacZa, Kmr, mcs mobilizable vector, allows for selection of double crossover C. glutamicum [1] pJYS1Ptac pBL1ts oriVC.glutamicum Kmr pSC101 oriVE. coli lacIq, PlacMFnCpf1, Ptac-RecT Addgene #85545 pJYS2_crtYf rep oriVC.glutamicum Spr pMB1 oriVE. .. Coli Pj23119-crRNA targeting crtYf Addgene #85544 pSL360 pSK1Cat carrying P180 [2] pHK432 Deletion of cpf1 gene from pJYS1Ptac This study pHK473 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtYf This study pHK475 Deletion of crRNA region from pHK473 This study pHK487 pK18mobsacB containing HR1 and the cpf1 gene for integration between cg1121 and cg1122 This study pHK489 pBL1ts oriVC.glutamicum Cmr pSC101 oriVE. coli lacIq, Ptac-RecT This study pHK493 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATAACATCGCCA160) This study pHK494 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTCTAACATCGCCA160) This study pHK495 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGGCTAACATCGCCA160) This study pHK496 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCCGCTAACATCGCCA160) This study pHK497 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATCACATCGCCA160) This study pHK498 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATCCCATCGCCA160) This study pHK499 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATCCAATCGCCA160) This study Table S2.
Selection:Article Title: Single-Base Genome Editing in Corynebacterium glutamicum with the Help of Negative Selection by Target-Mismatched CRISPR/Cpf1
Article Snippet: .. Name Characteristics Reference or source Bacterial strains Escherichia coli DH5α F-eNDA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17 (rK-mK),λ– Laboratory stock Corynebacterium glutamicum ATCC 13869 Wild type American Type Culture Collection (ATCC) HK1220 Single cross-over integration of the cpf1 gene in ATCC 13869 This study Plasmids pK18mobsacB sacB, lacZa, Kmr, mcs mobilizable vector, allows for selection of double crossover C. glutamicum [1] pJYS1Ptac pBL1ts oriVC.glutamicum Kmr pSC101 oriVE. coli lacIq, PlacMFnCpf1, Ptac-RecT Addgene #85545 pJYS2_crtYf rep oriVC.glutamicum Spr pMB1 oriVE. .. Coli Pj23119-crRNA targeting crtYf Addgene #85544 pSL360 pSK1Cat carrying P180 [2] pHK432 Deletion of cpf1 gene from pJYS1Ptac This study pHK473 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtYf This study pHK475 Deletion of crRNA region from pHK473 This study pHK487 pK18mobsacB containing HR1 and the cpf1 gene for integration between cg1121 and cg1122 This study pHK489 pBL1ts oriVC.glutamicum Cmr pSC101 oriVE. coli lacIq, Ptac-RecT This study pHK493 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATAACATCGCCA160) This study pHK494 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTCTAACATCGCCA160) This study pHK495 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGGCTAACATCGCCA160) This study pHK496 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCCGCTAACATCGCCA160) This study pHK497 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATCACATCGCCA160) This study pHK498 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATCCCATCGCCA160) This study pHK499 pBL1ts oriVC. glutamicum Spr pMB1 oriVE. coli Pj23119-crRNA targeting crtEb (140TTATCCCGTATCCAATCGCCA160) This study Table S2.
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